dnah2 64309 Search Results


primary antibodies dnah2 64309  (Thermo Fisher)
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Thermo Fisher primary antibodies dnah2 64309
(A) Proteins with significantly altered content in sperm from Cep76 mutants. (B) Proteins with significantly altered content in sperm from Cep76 mutants, after normalisation to alpha-tubulin content.
Primary Antibodies Dnah2 64309, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Proteins with significantly altered content in sperm from Cep76 mutants. (B) Proteins with significantly altered content in sperm from Cep76 mutants, after normalisation to alpha-tubulin content.

Journal: Life Science Alliance

Article Title: Genetic mutation of Cep76 results in male infertility due to abnormal sperm tail composition

doi: 10.26508/lsa.202302452

Figure Lengend Snippet: (A) Proteins with significantly altered content in sperm from Cep76 mutants. (B) Proteins with significantly altered content in sperm from Cep76 mutants, after normalisation to alpha-tubulin content.

Article Snippet: To define the localisation of a subset of differentially expressed proteins, fixed sperm were permeabilised in 0.2% Triton X-100/PBS, blocked in CAS-Block, incubated overnight in primary antibodies (0.5 μg/ml DNAH2 [64309; Invitrogen], 2.5 μg/ml SEPT4 [166788; Abcam]) at 4°C, stained with relevant fluorescent secondary antibodies (Thermo Fisher Scientific) for 1 h, then counterstained with DAPI.

Techniques: Protease Inhibitor, Ubiquitin Proteomics, Clinical Proteomics, Membrane

(A) Wild-type versus Cep76 mutant data for (A) DNAH2 localisation in cauda epididymal sperm and (B) AKAP4 localisation in cauda epididymal sperm. Scale bars = 20 μm. Arrows point to the accumulation of DNAH2 or AKAP4 in the neck region of sperm. (C, D) Number of sperm with this neck localisation was quantified, as shown in (C, D), for DNAH2 and AKAP4, respectively. (E, F) Average tail pixel intensity (per area) of DNAH2 and AKAP4 was quantified and is shown in (E, F). *** P < 0.001, ** P < 0.01, * P < 0.05. All n = 3–5.

Journal: Life Science Alliance

Article Title: Genetic mutation of Cep76 results in male infertility due to abnormal sperm tail composition

doi: 10.26508/lsa.202302452

Figure Lengend Snippet: (A) Wild-type versus Cep76 mutant data for (A) DNAH2 localisation in cauda epididymal sperm and (B) AKAP4 localisation in cauda epididymal sperm. Scale bars = 20 μm. Arrows point to the accumulation of DNAH2 or AKAP4 in the neck region of sperm. (C, D) Number of sperm with this neck localisation was quantified, as shown in (C, D), for DNAH2 and AKAP4, respectively. (E, F) Average tail pixel intensity (per area) of DNAH2 and AKAP4 was quantified and is shown in (E, F). *** P < 0.001, ** P < 0.01, * P < 0.05. All n = 3–5.

Article Snippet: To define the localisation of a subset of differentially expressed proteins, fixed sperm were permeabilised in 0.2% Triton X-100/PBS, blocked in CAS-Block, incubated overnight in primary antibodies (0.5 μg/ml DNAH2 [64309; Invitrogen], 2.5 μg/ml SEPT4 [166788; Abcam]) at 4°C, stained with relevant fluorescent secondary antibodies (Thermo Fisher Scientific) for 1 h, then counterstained with DAPI.

Techniques: Mutagenesis